支气管哮喘患者外周血CD3+T细胞趋化因子受体表达的研究

Research of chemokine receptors expression on CD3+T blood cells in bronchial asthma

摘要:

目的 探讨支气管哮喘患者外周血CD3⁺T细胞趋化因子受体表达情况。方法 选取2017年1月—2017年12月山东省立第三医院就诊的40例支气管哮喘患者及同期该院体检的27例健康志愿者，采用流式细胞仪检测所有受试者外周血CD3⁺CD8⁺和CD3⁺CD8^-T细胞上趋化因子受体CXCR1、CXCR2、CXCR3、CCR3、CCR4、CCR5、CCR7、CCR8的表达。比较轻、中度支气管哮喘患者、重度支气管哮喘患者及健康志愿者，以及不同剂量糖皮质激素使用者趋化因子受体表达的差异。结果 轻、中度支气管哮喘组、重度支气管哮喘组、对照组受试者CD3⁺CD8⁺T细胞上CXCR1、CCR7阳性表达率差异有统计学意义（P <0.05）。重度支气管哮喘组CD3⁺CD8⁺T细胞上CXCR1阳性表达率低于轻、中度支气管哮喘组和对照组（P <0.05）。重度支气管哮喘组CD3⁺CD8⁺T细胞上CCR7阳性表达率低于对照组（P <0.05）。轻、中度支气管哮喘组、重度支气管哮喘组、对照组受试者CD3⁺CD8^-T细胞上CXCR1、CXCR2阳性表达率差异有统计学意义（P <0.05）。重度支气管哮喘组CD3⁺CD8^-T细胞上CXCR1、CXCR2阳性表达率低于轻、中度支气管哮喘组和对照组（P <0.05）。3组吸入不同剂量糖皮质激素患者CD3⁺CD8⁺和CD3⁺CD8^-T细胞上CXCR1的阳性表达率差异有统计学意义（P <0.05）。每天吸入>800 μg/g布地奈德组的患者CD3⁺CD8⁺和CD3⁺CD8^-T细胞上CXCR1阳性表达率低于每天吸入< 400 μg/g布地奈德组的患者（P <0.05）。结论 重度支气管哮喘组患者外周血CD3⁺T细胞部分趋化因子表达减少，轻、中度哮喘患者Th1和Th2相关的趋化因子受体参与免疫应答，而重度支气管哮喘患者Th1/Th2应答下降。

Abstract:

Objective To analyze the pattern of chemokine receptor expression on T cells in bronchial asthma patients.Methods Forty patients with bronchial asthma who were admitted to our hospital from January to December 2017 and 27 healthy volunteers who underwent physical examination during the same period were included. Flow cytometric analysis of CXCR1, CXCR2, CXCR3, CCR3, CCR4, CCR5, CCR7, CCR8 expression on CD3⁺CD8⁺ and CD3⁺CD8^-T cells was performed in patients with different severity of chronic asthma and in the controls.Results Percentage of CD3⁺CD8⁺T cells expressing CXCR1 was significantly lower in severe asthmatic than in mild-to-moderate asthmatics and controls (P < 0.05). Percentages of CD3⁺CD8⁺T cells expressing CCR7 were significantly lower in the severe asthma group than in control group (P < 0.05). Percentages of CD3⁺CD8^-T cells expressing CXCR1, CXCR2, and CCR8 were significantly lower in the severe asthma group than in mild-to-moderate asthmatics and controls (all P < 0.05). The number of cells CD3⁺CD8⁺ and CD3⁺CD8^-T expressing of CXCR1 was significantly lower in the group of patients using more than 800 mg of budesonide daily than in the group of patients using less than 400 mg of budesonide (P < 0.05).Conclusions The expression of some chemokines of two CD3⁺T cell populations in peripheral blood of patients with severe bronchial asthma decreases. Th1 and Th2 related chemokine receptors are involved in immune response in patients with mild and moderate asthma, while Th1 / Th2 response in patients with severe bronchial asthma decreases.