RNF2在结直肠癌中的表达及其对结直肠癌细胞生物学行为的影响

Expression of RNF2 in colorectal cancer tissues and its effect on biological behavior of colorectal cancer cells

摘要:

目的 探讨环指蛋白2（RNF2）在结直肠癌中的表达及其对结直肠癌细胞生物学行为的影响。方法 采用免疫组织化学法检测RNF2在结直肠癌组织和癌旁正常组织中的表达，并利用过表达的慢病毒转染HCT116细胞构建过表达RNF2的HCT116细胞系，通过CCK-8法、划痕实验、Transwell实验检测过表达RNF2对结直肠癌细胞增殖、迁移、侵袭等生物学行为的影响。结果 结直肠癌组织中RNF2阳性表达率高于癌旁正常组织（P <0.05）；不同年龄、组织学类型、分化程度、Dukes分期、淋巴结转移的结直肠癌患者的RNF2表达阳性率比较，差异有统计学意义（P <0.05）；HCT116组、HCT116-control组、HCT116-RNF2组HCT116细胞增殖、迁移能力及侵袭能力比较，差异有统计学意义（P <0.05）；HCT116-RNF2组细胞增殖速度、迁移能力及侵袭能力均高于HCT116-control组和HCT116组（P <0.05）。结论 RNF2在结直肠癌中呈高表达，且过表达RNF2可促进结直肠癌细胞迁移、增殖、侵袭能力，可为结直肠癌治疗提供新的治疗靶点。

Abstract:

Objective To investigate Expression of ring finger protein 2 (RNF2) in colorectal cancer (CRC) tissues and its relationship with biological characteristics.Methods The expression of RNF2 in CRC tissues and adjacent normal tissues was detected by immunohistochemistry. RNF2-overexpressing HCT-116 colorectal cancer cell lines were constructed, then the effects of overexpression of RNF2 on proliferation, migration, and invasion of colorectal cancer cells were studied by CCK-8, scratch test and Transwell chamber.Results The positive expression rate of RNF2 in CRC tissues was higher than that in adjacent normal tissues (P < 0.05). The expression of RNF2 showed significant difference in CRC patients with different age, histological types, differentiation degree, Dukes stage, and lymph node metastasis (P < 0.05). HCT-116 cell proliferation rate, migration ability and invasive ability in HCT-116 group, HCT-116-control group, and HCT-116-RNF2 group respectively showed statistically significant differences (P < 0.05). The proliferation rate, migration ability, and invasion ability of HCT-116-RNF2 cells were higher than those of HCT-116-control and HCT-116 (P <0.05).Conclusion RNF2 is highly expressed in CRC, and the overexpression of RNF2 can promote the migration, proliferation, and invasion of CRC cells, which can be a new therapeutic target for CRC treatment.