幽门螺杆菌感染性胃癌组织microRNA-17、microRNA-490-3p的表达及其与临床病理特征和预后的关系

Relationship of microRNA-17 and microRNA-490-3p expression with clinicopathological characteristics and prognosis in patients with Helicobacter pylori induced gastric cancer

摘要:

目的 探讨幽门螺杆菌（Hp）感染性胃癌组织microRNA-17（miR-17）、microRNA-490-3p（miR-490-3p）的表达及其与临床病理特征和预后的关系。方法 选取2012年1月—2014年6月东莞市人民医院收治的146例Hp阳性及阴性胃癌患者的癌组织及癌旁组织。实时荧光定量聚合酶链反应（qRT-PCR）检测癌组织及癌旁组织中miR-17、miR-490-3p的表达。分析Hp阳性胃癌患者癌组织miR-17、miR-490-3p表达与临床病理特征的关系；Kaplan-Meier生存曲线评估miR-17、miR-490-3p表达与Hp阳性胃癌患者术后5年生存率的关系；Cox回归模型分析影响Hp阳性胃癌患者预后的危险因素。结果 Hp阳性及阴性胃癌患者的癌组织中miR-17 mRNA相对表达量高于癌旁组织（P <0.05），miR-490-3p mRNA相对表达量低于癌旁组织（P <0.05）。经Pearson相关分析，miR-17和miR-490-3p在不同胃癌组织中的表达无相关性（P >0.05）。不同肿瘤直径、淋巴结转移、远处转移及TNM分期的Hp阳性胃癌患者癌组织miR-17 mRNA相对表达量比较，差异有统计学意义（P <0.05）；不同肿瘤直径、淋巴结转移及远处转移的Hp阳性胃癌患者癌组织miR-490-3p mRNA相对表达量比较，差异有统计学意义（P <0.05）。Kaplan-Meier生存分析表明，miR-17高表达Hp阳性胃癌组、miR-490-3p低表达Hp阳性胃癌组术后5年生存率均分别低于miR-17低表达Hp阳性胃癌组、miR-490-3p高表达Hp阳性胃癌组（P <0.05）。有远处转移、较高的TNM分期、miR-17高表达、miR-490-3p低表达是影响Hp阳性胃癌患者预后的危险因素（P <0.05）。结论 Hp阳性胃癌组织miR-17表达增加、miR-490-3p表达降低，与患者较差临床病理参数和不良预后有关，监测癌组织miR-17、miR-490-3p表达可能有利于患者的预后判断。

Abstract:

Objective To investigate the relationship between the microRNA-17 (miR-17), microRNA-490-3p (miR-490-3p) expression with the clinicopathological characteristics and prognosis in patients with Helicobacter pylori (Hp) induced gastric cancer.Methods From January 2012 to June 2014, the gastric tissue samples from the Hp-positive and Hp-negative gastric cancer patients were collected, and the real time quantitative PCR (qRT-PCR) assay was used to detect the expression levels of miR-17, miR-490-3p in tissue samples. The relationships of miR-17 and miR-490-3p expression in Hp-positive gastric cancer with the clinicopathological characteristics were analyzed. The Kaplan Meier survival curve was utilized to evaluate the relationship of miR-17 and miR-490-3p expression with the 5-year overall survival rate of Hp positive gastric cancer patients. The Cox regression model was used to analyze the risk factors for prognostic of Hp positive gastric cancer patients.Results In the cancer tissues of Hp-positive and Hp-negative gastric cancer, the relative expression of miR-17 were higher than those in adjacent normal tissues (P < 0.05), the relative expression of miR-490-3p were lower than those in adjacent normal tissues (P < 0.05). Pearson correlation test showed that there was no significant correlation between the expression of miR-17 and miR-490-3p in gastric cancer tissue (P > 0.05). In Hp-positive gastric cancer tissues, there were statistical significance of the miR-17 expressions compared in different samples of patients with tumor diameter, lymph node metastasis, distance metastasis, and TNM stages (P < 0.05), there were statistical significance of the miR-490-3p expressions in samples of patients with different tumor diameter, lymph node metastasis, and distance metastasis (P < 0.05). Kaplan-Meier survival analysis showed that the 5-year overall survival rates of the miR-17 high-expression Hp-positive gastric cancer group and the miR-490-3p low-expression Hp-positive gastric cancer group were lower than those of the miR-17 low-expression Hp-positive gastric cancer group and the miR-490-3p high-expression Hp-positive gastric cancer group, respectively (P < 0.05). Multivariate Cox regression model suggests that, distance metastasis, high TNM stage, miR-17 high-expression, and miR-490-3p low-expression were risk factors affecting the prognosis of gastric cancer patients.Conclusion In HP-positive gastric cancer tissues, the miR-17 expression are increased, and miR-490-3p expression are decreased, which are related to worse clinicopathological parameters and poor prognosis. Monitoring the miR-17 and miR-490-3p expression in the cancer tissues may be useful in predicting the prognosis of patients.