肺炎支原体肺炎患儿血清microRNA-21和microRNA-221水平变化及与炎症因子、T淋巴细胞亚群的相关性

Changes in serum microRNA-21 and microRNA-221 levels in children with mycoplasma pneumoniae and their relationship with inflammatory factors and T lymphocyte subset

摘要:

目的 探讨肺炎支原体肺炎（MPP）患儿血清microRNA-21（miR-21）和microRNA-221（miR-221）的水平变化及与炎症因子、T淋巴细胞亚群的关系。方法 选取2017年1月—2019年1月邯郸市中心医院就诊的MPP患儿120例为研究对象（MPP组），另选取本院同期体检的健康儿童100例为对照组（CON组）。比较两组研究对象血清miR-21和miR-221的水平差异，并分析二者与患儿外周血中炎症因子［白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）］和T淋巴细胞亚群（CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺）之间的相关性。结果 MPP组血清miR-21和miR-221水平高于CON组（P <0.05）。MPP组外周血CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺低于CON组，而IL-6、IL-8、TNF-α的水平高于CON组（均P <0.05）。MPP组血清miR-21和miR-221与患者外周血CD3⁺（r =-0.494和-0.656，P =0.037和0.014）、CD4⁺（r =-0.621和-0.554，P =0.015和0.031）、CD8⁺（r =-0.772和-0.476，P =0.003和0.043）、CD4⁺/CD8⁺（r =-0.545和-0.660，P =0.023和0.014）均呈负相关，而与TNF-α（r =0.787和0.619，P =0.000和0.015）、IL-6（r =0.530和0.598，P =0.035和0.029）、IL-8（r =0.665和0.614，P =0.012和0.017）均呈正相关。结论 MPP患儿血清miR-21和miR-221水平升高，且与患儿T淋巴细胞亚群水平的降低、炎症因子水平的升高有一定的相关性，监测两者的水平变化有助于评估MPP患儿的免疫状态和炎症反应，并为合理制订治疗方案提供帮助。

Abstract:

Objective To study the changes of serum microRNA-21(miR-21) and microRNA-221(miR-221)levels in children with mycoplasma pneumoniae pneumonia (MPP)and their relationship with inflammatory factors and T lymphocyte subsets.Methods From January 2017 to January 2019, 120 children with MPP who were treated in our hospital were selected as the study objects (MPP group), and 100 healthy children who were examined in the same period were selected as the control group (CON group). The levels of miR-21 and miR-221 in serum of the two groups were compared, and the correlation between them and inflammatory factors [Interleukin (IL) - 6, IL-8, Tumor necrosis factor - α (TNF - α)] and T lymphocyte subsets (CD3⁺, CD4⁺, CD8⁺, CD4⁺/ CD8⁺) in peripheral blood of children was analyzed.Results The serum levels of miR-21 and miR-221 in the MPP group were higher than those in the CON group (all P < 0.05). Levels of CD3⁺, CD4⁺, CD8⁺, and CD4⁺/ CD8⁺ in peripheral blood of the MPP group were lower than those of the CON group, while levels of TNF-α, IL-6, and IL-8 were higher than those of the CON group (all P < 0.05). Serum miR-21 and miR-221 in the MPP group were negatively correlated with CD3⁺ (r = -0.494, P = 0.037; r = -0.656, P = 0.014), CD4⁺ (rr =-0.621, P = 0.015; r=-0.554, P = 0.031), CD8⁺ (r = -0.772, P = 0.003; r = -0.476, P =0.043), and CD4⁺/CD8⁺ (r = -0.545, P = 0.023; r = -0.660, P = 0.014) in the peripheral blood of the patients, while significantly positively correlated with TNF-α (r =0.787, P = 0.000; r =0.619, P = 0.015), IL-6 (r = 0.530, P = 0.035; r = 0.598, P = 0.029), and IL-8 (r = 0.665, P = 0.012; r = 0.614, P = 0.017).Conclusion The levels of miR-21 and miR-221 in MPP children's serum are increased, which have a certain correlation with the decrease of T-lymphocyte subsets and the increase of inflammatory factors. Monitoring the changes of the levels of two indexes is helpful to evaluate the immune status and inflammatory response of MPP children, and to provide help for the rational development of treatment programs.