MicroRNA-122-5p、microRNA-33a-3p在胃癌患者中的表达及与临床病理特征和预后的关系

Relationship of miR-122-5p and miR-133a-3p with clinicopathological parameters and prognosis in patients with gastric cancer

摘要:

目的 探究胃癌患者血清microRNA-122-5p（miR-122-5p）、microRNA-133a-3p（miR-133a-3p）mRNA相对表达量与临床病理特征及预后的关系。方法 采集胃癌患者血清标本94例，以相同例数的健康体检者的血清样本作为对照。采用实时荧光定量聚合酶链反应（qRT-qPCR）技术检测血清miR-122-5p、miR-133a-3p mRNA相对表达量，同时分析两者与胃癌患者临床病理特征的关系；绘制Kaplan-Meier生存曲线，分析miR-122-5p、miR-133a-3p的表达与胃癌患者预后的关系。结果 胃癌患者血清miR-122-5p、miR-133a-3p mRNA相对表达量低于健康体检者（P <0.05）；有淋巴结转移、分化程度低、浸润至浆膜层的患者血清miR-122-5p mRNA相对表达量低于无淋巴结转移，中、高分化程度、浸润至黏膜下各层的患者（P <0.05）；有淋巴结转移、低分化程度、浸润至浆膜层、TNM分期为Ⅲ+Ⅳ期的患者血清miR-133a-3p mRNA相对表达量低于无淋巴结转移，中、高分化程度，浸润至黏膜下各层，TNM分期为Ⅰ+Ⅱ期的患者（P <0.05）；Kaplan-Meier生存曲线结果显示，miR-122-5p低表达胃癌患者5年生存率低于miR-122-5p高表达胃癌患者（P <0.05）；miR-133a-3p低表达胃癌患者的5年生存率低于miR-133a-3p高表达胃癌患者（P <0.05）。结论 胃癌患者血清miR-122-5p、miR-133a-3p mRNA相对表达量呈低表达，并且两者与胃癌的恶性程度和患者不良预后密切相关，血清miR-122-5p、miR-133a-3p可能成为胃癌患者预后预测的潜在生物标志物。

Abstract:

Objective To investigate the expression levels of miR-122-5p and miR-133a-3p in the serum of patients with gastric cancer, and to analyze the relationship of the two with the clinicopathological parameters and prognosis of patients with gastric cancer.Methods Serum samples from 94 patients with gastric cancer were collected, and serum samples from healthy volunteers with the same number were used as controls. Real-time quantitative PCR (qRT-PCR) was used to detect the expression levels of miR-122-5p and miR-133a-3p in serum samples, and the relationship between the two and clinical pathological parameters of gastric cancer patients was analyzed; Kaplan-Meier survival curve analysis was used; relationship of miR-122-5p and miR-133a-3p with the prognosis of patients with gastric cancer was analyzed.Results The expression levels of miR-122-5p and miR-133a-3p in the serum of patients with gastric cancer were significantly lower than those in healthy volunteers (P < 0.05). The serum miR-122-5p level in patients with lymph node metastasis, low differentiation, invasion of serous layer was lower than that in patients with none-lymph node metastasis, moderate and high differentiation, invasion of layers under mucous layer (P < 0.05), while the serum mir-133a-3p level in patients with lymph node metastasis, low differentiation, invasion of serous layer, III+IV TNM stage was lower than that in patients with none-lymph node metastasis, moderate and high differentiation, invasion of layers under mucous layer, I+II TNM stage (P < 0.05). Kaplan Meier survival curve showed that the 5-year survival rate of patients in miR-122-5p low expression gastric cancer was lower than that of patients in miR-122-5p high expression gastric cancer (P < 0.05). The 5-year survival rate of patients in miR-133a-3p low expression gastric cancer was significantly lower than that of patients in miR-133a-3p high expression gastric cancer (P < 0.05).Conclusion The expression levels of miR-122-5p and miR-133a-3p in serum of patients with gastric cancer are low, and they are closely related to the malignant degree of gastric cancer and the poor prognosis of patients. MiR-122-5p and miR-133a-3p may become a potential biomarker for the prognosis of gastric cancer patients.