胃癌组织SOX1和β-catenin的表达及其与临床病理特征、远期预后的相关性

Expression of SOX1 and β-catenin in gastric cancer and its relationship with pathological characteristics and long-term prognosis

摘要:

目的 探讨胃癌组织性别决定区Y框蛋白1（SOX1）和β-连环蛋白（β-catenin）的表达及其与临床病理特征、远期预后的相关性。方法 收集2015年2月—2017年3月在西安市第四医院行胃癌根治术或姑息性切除术患者的胃癌组织及癌旁组织，采用免疫组织化学染色检测SOX-1、β-catenin的阳性表达；Western blotting检测SOX-1、β-catenin蛋白相对表达量；随访患者的总体生存情况。结果 胃癌组织SOX1的阳性表达率低于癌旁组织（P <0.05），β-catenin的阳性表达率高于癌旁组织（P <0.05）；胃癌组织SOX1蛋白相对表达量低于癌旁组织（P <0.05），β-catenin蛋白相对表达量高于癌旁组织（P <0.05）；胃癌组织SOX1蛋白相对表达量与β-catenin蛋白相对表达量呈正相关（r =0.347，P =0.017）；低分化，TNM Ⅲ、Ⅳ期，有淋巴结转移胃癌组织SOX1的阳性表达率低于中、高分化，TNMⅠ、Ⅱ期，无淋巴结转移胃癌组织（P <0.05）；低分化，TNMⅢ、Ⅳ期，有淋巴结转移胃癌组织β-catenin的阳性表达率高于中、高分化，TNMⅠ、Ⅱ期，无淋巴结转移胃癌组织（P <0.05）；与SOX1阴性表达患者比较，SOX1阳性表达患者的总生存期延长（P <0.05）；与β-catenin阴性表达患者比较，β-catenin阳性表达患者的总生存期缩短（P <0.05）。结论 胃癌组织中SOX1的低表达与β-catenin的高表达有关且与临床病理特征、远期预后的恶化相关。

Abstract:

Objective To study the relationship between the expression of sex determining region Y-box 1(SOX1) and β-catenin in gastric cancer and its correlation with pathological characteristics and long-term prognosis.Methods Gastric cancer tissues and the adjacent tissues which were excised in our hospital during February 2015 to March 2017 were collected. The expression of SOX1 and β-catenin were detected by immunohistochemistry and Western blotting, and the overall survival of the patients was followed up.Results In gastric cancer, the positive rate of SOX1 was significantly lower than those in adjacent tissues (P < 0.05), the positive rate of β-catenin was significantly higher than those in adjacent tissues (P < 0.05), the relative expression of SOX1 was significantly lower than those in adjacent tissues (P < 0.05), the relative expression of β-catenin was significantly higher than those in adjacent tissues (P < 0.05), and the relative expression of SOX1 in gastric cancer was positively correlated with the relative expression of β-catenin (r = 0.347, P = 0.017); in poorly differentiated, TNM III / IV stage, and lymph node metastasis gastric cancer tissues, the positive expression rate of SOX1 was significantly lower than that in middle or high differentiation, TNM I / II stage, and non lymph node metastasis gastric cancer, and the positive expression rate of β-catenin was significantly higher than that in middle and high differentiation, TNM I, II stage, non lymph node metastasis gastric cancer (P < 0.05). Compared with patients with negative expression of SOX1, the overall survival of patients with positive expression of SOX1 was prolonged (P < 0.05); compared with patients with negative expression of β-catenin, the overall survival of patients with positive expression of β-catenin was shorten (P < 0.05).Conclusion The low expression of SOX1 in gastric cancer is related to the high expression of β-catenin, and correlate to pathological characteristics and long-term prognosis.