超声造影评价地塞米松治疗大鼠肾脏缺血再灌注损伤的实验研究

Experimental research on evaluation value of contrast-enhanced ultrasound for dexamethasone on renal ischemia-reperfusion injury in rats

摘要:

目的 探讨超声造影在评价地塞米松治疗大鼠肾脏缺血再灌注损伤中的应用价值。方法 72只健康雄性SD大鼠，随机分为假手术组、缺血再灌注组、地塞米松组，每组24只。3组大鼠分别再灌注1 d、3 d、5 d、7 d，每次各6只。超声造影后，分析3组大鼠再灌注不同时间点TIC曲线中峰值强度（Peak）、达峰时间（TP）、平均渡越时间（MTT）、曲线下面积（AUC）的变化，比较3组大鼠肾脏病理改变，再灌注不同时间点的血肌酐、尿素氮水平。结果 3组大鼠在不同再灌注时间点的肾皮质TIC曲线各参数比较，经析因设计的方差分析，①不同再灌注时间点的Peak、TP、MTT、AUC有差异（P <0.05）；②3组大鼠的Peak、TP、MTT、AUC有差异（P <0.05）；③3组大鼠的Peak、TP、MTT、AUC随再灌注时间的变化趋势有差异（P <0.05）。与假手术组比较，缺血再灌注组肾脏病理损伤加重，而地塞米松组损伤有缓解。3组大鼠血肌酐、尿素氮水平比较，经析因设计的方差分析，①不同再灌注时间点的血肌酐、尿素氮水平有差异（P <0.05）；②3组大鼠的血肌酐、尿素氮水平有差异（P <0.05）；③3组大鼠的血肌酐、尿素氮水平随再灌注时间的变化趋势有差异（P <0.05）。结论 超声造影可以定量反映大鼠肾脏缺血再灌注后不同时间段及地塞米松干预后肾脏微循环的改变，其中TP具有较大参考价值。

Abstract:

Objective To investigate the value of contrast-enhanced ultrasound in monitoring dexamethasone for renal Ischemia reperfusion injury in rats.Methods Totally 72 healthy male SD rats were randomly divided into Sham group (Sham), IRI group (IRI), and Dexa group (Dexa), 24 rats in each group. According to the different reperfusion time points, they were divided into 1 d, 3 d, 5 d, and 7 d groups, with 6 rats in each group. After contrast-enhanced ultrasound, the changes in TIC Peak, TP, MTT, and AUC in different time periods and after Dexa treatment were analyzed and compared with Crea, Urea nitrogen, and pathological changes.Results The results showed that: (1) The Peak, TP, MTT, and AUC were different at different reperfusion time points (P <0.05); (2) There were differences in Peak, TP, MTT, and AUC among the three groups (P < 0.05). (3) The Peak, TP, MTT, and AUC of the three groups were different with the time of reperfusion (P < 0.05). Compared with the sham operation group, the renal pathological injury was aggravated in the ischemia-reperfusion group, while the injury was alleviated in the dexamethasone group. The levels of serum creatinine and urea nitrogen were compared among the three groups. The results showed that: (1) The levels of serum creatinine and urea nitrogen were different at different reperfusion time points (P < 0.05). (2) There were differences in serum creatinine and urea nitrogen levels among the three groups (P <0.05); (3) The changes of serum creatinine and urea nitrogen in the three groups were different with the time of reperfusion (P < 0.05). Pairwise comparison between groups using the least significant difference method found that only TP values were statistically significant at each time period (P < 0.05).Conclusions Contrast-enhanced ultrasound technique can quantitatively reflect the changes of renal microcirculation after renal ischemia reperfusion in rats at different time periods and after dexamethasone intervention, of which TP has great reference value.