七氟醚对结直肠癌细胞侵袭迁移的影响及对microRNA-203的调控作用机制研究

Effect of sevoflurane on invasion and migration of colorectal cancer cells based on ERK/MMP-9 signaling pathway and its regulatory effect on miR-203

摘要:

目的 观察七氟醚对结直肠癌细胞侵袭迁移的影响及对microRNA-203（miR-203）的调控作用，探讨其可能作用机制。方法 将HCT116细胞分为对照组（5%CO₂培养+未转染）、七氟醚组（4%七氟醚+5%CO₂培养+未转染），miR-203组（5%CO₂培养+转染miR-203 mimics）、miR-203+七氟醚组（4%七氟醚+5%CO₂培养+转染miR-203 mimics），miR-203-NC+七氟醚组（4%七氟醚+5% CO₂培养+转染miR-203 mimics-NC）。CCK-8实验检测细胞增殖能力；qRT-PCR法检测细胞的miR-203表达；划痕实验检测细胞迁移能力；Transwell实验检测细胞侵袭能力；Western blotting法检测细胞外信号调节激酶（ERK）、p-ERK、基质金属蛋白酶-9（MMP-9）蛋白水平。结果 miR-203+七氟醚可抑制细胞增殖，降低细胞迁移能力，减少穿膜细胞数，降低细胞中p-ERK、MMP-9蛋白表达。结论 七氟醚可抑制HCT116细胞侵袭、迁移，可能是通过上调miR-203表达、阻断ERK/MMP-9信号通路发挥作用。

Abstract:

Objective To observe the effect of sevoflurane on the invasion and migration of colorectal cancer cells and the regulation of miR-203, and to explore its possible mechanism.Methods HCT116 cells were divided into control group (5% CO₂ culture + untransfected), sevoflurane group (4% sevoflurane + 5% CO₂ culture +untransfected), miR-203 group (5% CO₂ culture + transfection with miR-203 mimics), miR-203+ sevoflurane group (4% sevoflurane + 5% CO₂ culture+ transfection with miR-203 mimics), miR-203-NC+ sevoflurane group (4% sevoflurane) Ether + 5% CO₂ culture+ transfection miR-203 mimics-NC). CCK-8 method was used to detect cell proliferation; qRT-PCR was used to detect the expression of miR-203 in cells; scratch test was used to detect cell migration ability; Transwell test was used to detect cell invasion ability; Western blotting method was used to detect extracellular signal-regulated kinase (ERK), p-ERK, and matrix metalloproteinase-9 (MMP-9) protein levels.Results MiR-203+ sevoflurane can inhibit cell proliferation and reduce cell migration ability, the number of penetrating cells, and the expression of p-ERK and MMP-9 proteins in cells.Conclusion Sevoflurane can inhibit the invasion and migration of HCT116 cells, possibly with up-regulating the expression of miRNA-203 and blocking the ERK/MMP-9 signaling pathway.