先天性巨结肠患儿不同肠管中SOX-2、SOX-10、GDNF的表达及其临床意义

Expression and significance of SOX -2, SOX -10 and GDNF in different intestines of children with congenital megacolon

摘要:

目的 探讨先天性巨结肠患儿不同肠管中SOX-2、SOX-10、GDNF的表达及意义。方法 选取2014年1月—2019年8月邢台市人民医院手术治疗的先天性巨结肠患儿85例作为观察组，取切除的狭窄段、扩张段和移行段肠管组织，将同期行结肠造瘘术的患儿20例作为对照组，采用实时荧光定量聚合酶链反应（qRT-PCR）检测肠管组织SOX-2、SOX-10、GDNF的表达。结果 各组肌间神经丛直径、神经节细胞直径、神经节细胞数、神经节细胞质/核比较，差异有统计学意义（P <0.05）。其中，肠肌间神经丛直径：HD组狭窄段< HD组移行段< HD组扩张段和对照组；神经节细胞直径、神经节细胞数、神经节细胞质/核：HD组移行段< HD组扩张段和对照组；各组SOX-2、SOX-10、GDNF mRNA的相对表达量比较，差异有统计学意义（P <0.05），HD组狭窄段< 病例组移行段< HD组扩张段和对照组。对照组与HD组扩张段比较，差异无统计学意义（P >0.05）。结论 先天性巨结肠患儿病变肠管组织中SOX-2、SOX-10、GDNF mRNA的相对表达量在扩张段、移行段、狭窄段逐渐降低，这与病变肠神经系统发育异常有关。

Abstract:

Objective To investigate the expression and significance of SOX-2, SOX-10 and GDNF in the different intestinal tracts of children with congenital megacolon disease (HD).Methods Seleced 85 cases of HD children in our hospital from January 2014 to August 2019 who underwent surgical treatment as the observation group, of which the intestinal canal tissues of the narrowed, lated, and transitional segments were removed for experiment. At the same time, 20 cases of children undergoing colostomy were used as the control group. The fluorescent quantitative PCR was used to detect the mRNA levels of the SOX-2, the SOX-10, and the GDNF in the intestinal tissue.Results The diameter of myenteric nerve plexus, ganglion cell diameter, ganglion cell number, ganglion cytoplasm/nucleus ratio were compared in each group, and the difference was statistically significant after analysis of variance (all P < 0.05). Among them, the diameter of the intestinal myenteric plexus: HD group stenosis segment < HD group transitional segment < HD group expansion segment and control group; ganglion cell diameter, ganglion cell number, ganglion cytoplasm/nucleus ratio: HD group transitional segment < HD The expansion segment of the group and the control group. Comparison of the relative expression of SOX-2, SOX-10, GDNF at the mRNA level of each group showed statistically significant differences (P < 0.05). Among them, the relative expression of SOX-2, SOX-10, and GDNF at the mRNA level: stenosis segment in HD group < transition segment in case group < expanded segment in HD group, and the difference was statistically significant (P < 0.05). There was no significant difference between the control group and the HD group in the expansion segment (P > 0.05).Conclusion The expression levels of SOX-2, SOX-10, and GDNF in the pathological bowel tissues of HD children were gradually reduced in the dilatation segment, transitional segment, and stenosis segment, which were related to abnormal development of the pathological bowel nervous system.