1.兴安盟人民医院， 内蒙古 乌兰浩特 137400;2.内蒙古医科大学， 内蒙古 呼和浩特 010059;3.内蒙古医科大学基础医学院 微生物与免疫学研究室， 内蒙古 呼和浩特 010059
赵鹏伟，E-mail：pengwzhao@126.com
R737.31
1.Department of Clinical Laboratory, Xinganmeng Peoples' Hospital, Ulanhot, Inner Mongolia 137400, China;2.Inner Mongolia Medical University, Hohhot, Inner Mongolia 010059, China;3.Department of Pathogenic and Immunology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010059, China
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目的 分析二代测序技术ghrelin作用后的人卵巢癌细胞株SK-OV-3中长链非编码RNA（lncRNA）的差异表达。方法 从对照组（不添加ghrelin）和实验组（添加600 ng/mL ghrelin 24 h）的人卵巢癌细胞株SK-OV-3中分别提取RNA进行测序，筛选出实验组发生差异表达的lncRNA，对其进行靶基因预测并将预测结果与差异mRNA取交集，对取交集后的基因进行GO分析和KEGG通路分析。结果 筛选获得差异表达的lncRNA有236个（上调130个，下调106个）；差异表达mRNA有71个（上调66个，下调5个）。差异表达lncRNA靶基因与差异表达mRNA取交集筛选获得57个基因。GO和KEGG通路富集分析结果显示：这些差异表达lncRNA主要与精、赖氨酸跨膜转运，氧化应激反应及发育过程相关，并且主要富集在细胞因子受体信号通路、胰高血糖素和胰岛素信号通路及癌症相关信号通路上。结论 ghrelin作用后的人卵巢癌细胞中lncRNA的表达有差异，其可能参与卵巢癌的发生、发展，提示ghrelin可能在卵巢癌治疗中具有重要作用。
Objective To analyze the differential expression of long non coding RNA (long non-coding RNA, lncRNA) in human ovarian cancer cell line SK-OV-3 after treated with ghrelin.Methods Human ovarian cancer cell line SK-OV-3 RNA was extracted from the control group (without ghrelin) and the experimental group (with 600 ng / mL ghrelin for 24 h) for sequencing. Comparing the two groups, lncRNAs differentially expressed in the experimental group were screened to predict their target genes and intersect with differential mRNAs. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were carried out for the genes after the intersection.Results There were 236 differentially expressed lncRNAs, of which 130 were up-regulated and 106 were down-regulated. There were 71 differentially expressed mRNAs, of which 66 were up-regulated and 5 were down-regulated. 57 genes were screened by intersection of differentially expressed lncRNA target genes and differentially expressed mRNA. GO analysis showed that these lncRNAs were associated with L-lysine and arginine transmembrane transporter activity, response to oxygen level, response to stress and developmental process. KEGG pathway analysis showed that these lncRNAs were mainly enriched on the pathways, such as cytokine-cytokine receptor interaction, Glucagon signal pathway, Insulin signal pathway and pathway in cancer.Conclusion The expression of lncRNAs in human ovarian cancer cells treated with ghrelin are significantly different, which may be involved in the occurrence and development of ovarian cancer, suggesting that ghrelin may play an important role in the treatment of ovarian cancer.
引用本文
宋婉莹,萨日盖,高海宁,赵鹏伟.基于二代测序技术ghrelin作用后人卵巢癌细胞差异表达lncRNA的生物信息学分析[J].中国现代医学杂志,2022,(9):25-31
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- 收稿日期:2021-12-19
- 在线发布日期: 2023-10-30
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