产妇多环芳烃暴露水平与子代8-羟基脱氧鸟苷水平的相关性

Study on correlation between maternal exposure to polycyclic aromatic hydrocarbons and 8-hydroxydeoxyguanosinelevel in offspring

摘要:

目的 探讨产妇多环芳烃（PAHs）暴露水平与子代8-羟基脱氧鸟苷（8-OHdG）水平的关系。方法 选取新疆医科大学第五附属医院采暖期（2018年12月—2019年3月）和非采暖期（2019年6月—2019年10月）产妇各90例及其新生儿作为研究对象。对产妇进行流行病学调查；通过酶水解-液相色谱-质谱联用仪检测产妇尿液中1-羟基芘（1-OHP）、2-羟基萘（2-OHN）、9-羟基菲（9-OHPhe）、3-羟基苯并（a）芘（3-OHBaP）的水平；采用酶联免疫吸附试验（ELISA）检测新生儿脐带血中8-OHdG水平；采用Pearson法分析产妇与新生儿PAHs与8-OHdG的相关性。结果 采暖期组产妇尿液中1-OHP、2-OHN、3-OHBaP及新生儿血清8-OHdG的水平均高于非采暖期组（P <0.05）；两组产妇尿液中9-OHPhe比较，差异无统计学意义（P >0.05）。Pearson相关分析显示，产妇尿液中2-OHN（r =0.215）、3-OHBaP（r =0.199）与新生儿血清8-OHdG呈正相关（均P <0.05），产妇尿液中1-OHP（r =0.027）、9-OHPhe（r =0.097）与新生儿血清8-OHdG无相关性（均P >0.05）。结论 乌鲁木齐市孕妇多环芳烃暴露可能致新生儿DNA氧化损伤。

Abstract:

Objective To explore the relationship between maternal polycyclic aromatic hydrocarbons (PAHs) exposure and 8-hydroxydeoxyguanosine (8-OHdG) levels in offspring.Methods Maternal and newborn in the heating period (December 2018 to March 2019) and the non-heating period (20 June 2019 to October 2019) of the Fifth Affiliated Hospital of Xinjiang Medical University in Urumqi City were selected as the research objects. The epidemiological investigation of the parturient was carried out; the levels of 1-OHP, 2-OHN, 9-OHPhe, and 3-OHBaP in the urine of the parturient were detected by enzymatic hydrolysis-liquid chromatography-mass spectrometry; the enzyme-linked immunosorbent assay was used (ELISA) to detect the level of 8-OHdG in neonatal cord serum; Pearson correlation was used to analyze the correlation between PAHs and 8-OHdG in maternal and neonates.Results The urine levels of 1-OHP, 2-OHN, 3-OHBaP, and neonatal serum 8-OHdG in the heating period group were higher than those in the non-heating period group (P < 0.05); compared with 9-OHPhe, the difference was not statistically significant (P > 0.05). Pearson correlation analysis showed that 2-OHN (r = 0.215) and 3-OHBaP (r = 0.199) in the urine of parturients were positively correlated with neonatal serum 8-OHdG (P < 0.05). There was no correlation between 1-OHP (r = 0.027) and 9-OHPhe (rr = 0.097) in urine and 8-OHdG in neonatal serum (P > 0.05).Conclusions Exposure to polycyclic aromatic hydrocarbons in pregnant women in Urumqi may cause oxidative damage to neonatal DNA.