糖尿病患者血清microRNA-152表达水平与血脂代谢、胰岛素抵抗的相关性

The correlation of serum microRNA-152 expression with lipid metabolism and insulin resistance in patients with diabetes mellitus

摘要:

目的 探讨糖尿病患者血清microRNA-152（miR-152）表达水平与血脂代谢、胰岛素抵抗的相关性。方法 选取2018年1月—2018年11月在攀枝花市中西医结合医院就诊的血糖异常患者109例，分为2型糖尿病（T2DM）前期组（Pre-T2DM组）45例和T2DM组64例；另取同期该院门诊部健康体检者70例为对照组。检测血清空腹胰岛素（FINS）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、空腹血糖（FBG）水平，评估稳态胰岛素评价指数（HOMA-IR）与胰岛β细胞功能指数（HOMA-β）；并分析miR-152与血脂水平及FBG、FINS、HOMA-IR、HOMA-β的相关性。结果 与对照组相比，Pre-T2DM组、T2DM组患者血清miR-152、FBG、FINS、TG、TC、LDL-C、HOMA-IR较高（P <0.05），HDL-C、HOMA-β较低（P <0.05）；与Pre-T2DM组相比，T2DM组患者血清miR-152、FBG、FINS、TG、TC、LDL-C、HOMA-IR较高（P <0.05），HDL-C、HOMA-β较低（P <0.05）。T2DM患者miR-152与TG、TC、LDL-C、FBG、FINS、HOMA-IR呈正相关（r =0.844、0.755、0.833、0.528、0.576和0.635，均P <0.05），与HDL-C、HOMA-β呈负相关（r =-0.655和-0.603，均P <0.05）。多因素一般Logistic回归分析结果显示，血清FBG ［R=2.305（95% CI：1.369，3.882）］和miR-152 ［R =2.831（95% CI：1.736，4.618）］升高是影响胰岛素抵抗的危险因素。结论 T2DM患者血清miR-152上调，其可能通过调节血脂代谢影响T2DM病情发展。

Abstract:

Objective To investigate the correlation of serum miR-152 expression with glycolipid metabolism and insulin resistance in diabetic patients.Methods A total of 109 patients with abnormal blood sugar in our hospital from January to November 2018 were selected and divided into pre-T2DM (type 2 diabetes mellitus) group (n = 45) and T2DM group (n = 64). At the same time, 70 healthy people in outpatient department of our hospital were selected as control group. The serum levels of fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG), and miR-152 were measured. The homeostasis model assessment of insulin resistance (HOMA-IR) and β-cell function (HOMA-β) were evaluated. Besides, the correlations of miR-152 with blood lipid, FBG, FINS, HOMA-IR and HOMA-β were analyzed.Results Compared with the control group, the levels of serum miR-152, FBG, FINS, TG, TC and LDL-C as well as HOMA-IR value were higher in pre-T2DM group and T2DM group (P < 0.05), while the serum levels of HDL-C and HOMA-β value were lower (P < 0.05). Compared with the pre-T2DM group, the levels of serum miR-152, FBG, FINS, TG, TC and LDL-C as well as HOMA-IR value were higher in the T2DM group (P < 0.05), while the serum levels of HDL-C and HOMA-β value were lower (P < 0.05). Further analysis showed that miR-152 positively correlated with TG (r = 0.844), TC (r = 0.755), LDL-C (r = 0.833), FBG (r = 0.528), FINS (r = 0.576) and HOMA-IR (r = 0.635) (P < 0.05), but negatively correlated with HDL-C (r = -0.655) and HOMA-β (r = -0.603) in patients with T2DM (P < 0.05). Multivariate Logistic regression analysis indicated that the high level of serum miR-152 [R = 2.831 (95% CI: 1.736, 4.618)] was a risk factor for insulin resistance.Conclusions The level of serum miR-152 is up-regulated in patients with T2DM, which may affect the development of T2DM by regulating lipid metabolism.