支气管哮喘合并肺炎支原体感染患儿血清CysLTs、IL-13及IgE的表达水平

Expression levels of CysLTs, IL-13 and IgE in children with bronchial asthma complicated with Mycoplasma pneumoniae infection

摘要:

目的 探讨支气管哮喘患儿合并肺炎支原体感染时体内相关免疫因子半胱氨酰白三烯（CysLTs）、白细胞介素-13（IL-13）及免疫球蛋白E（IgE）的表达水平。方法 选取2019年1月—2020年1月台州市中心医院儿内科收治的136例支气管哮喘患儿作为研究对象，根据患儿是否合并肺炎支原体感染分为感染组（36例）与非感染组（100例）。以随机抽样的方法另取同期该院健康体检儿童35例作为对照组。采用酶联免疫吸附试验检测所有研究对象清晨空腹状态下血清CysLTs、IL-13及IgE水平。结果 3组患儿血清CysLTs、IL-13、IgE水平比较，差异有统计学意义（P <0.05）；感染组血清CysLTs、IL-13、IgE水平高于非感染组和对照组（P <0.05），非感染组CysLTs、IL-13、IgE水平高于对照组（P <0.05）。逐步多因素Logistic回归分析结果显示，CysLTs ［O^R=1.012（95% CI：1.008，1.025）］、IL-13 ［O^R=2.301（95% CI：1. 563，3.405）］、IgE ［O^R=3.921（95% CI：1.873，8.614）］是支气管哮喘患儿合并肺炎支原体感染的危险因素（P <0.05）。感染组CysLTs与IL-13、IgE呈正相关（r =0.979和0.992，P =0.000和0.012），IL-13与IgE呈正相关（r =0.931，P =0.000）。结论 支气管哮喘合并肺炎支原体感染患儿血清CysLTs、IL-13、IgE水平显著升高。

Abstract:

Objective To investigate the expression levels of cysteinyl leukotrienes (CysLTs), interleukin-13 (IL-13) and IgE in children with bronchial asthma complicated with Mycoplasma pneumoniae infection.Methods A total of 136 children with bronchial asthma admitted to the Department of Pediatrics of Taizhou Central Hospital from January 2019 to January 2020 were selected as the subjects. According to the presence or absence of Mycoplasma pneumoniae infection, they were divided into infection group (36 cases) and non-infection group (100 cases). Another 35 healthy children who underwent physical examinations at the Department of Pediatrics of Taizhou Central Hospital during the same period were selected as the control group. Enzyme-linked immunosorbent assay was used to detect the levels of CysLTs, IL-13 and IgE in serum samples of all subjects under fasting conditions in the morning.Results The levels of CysLTs, IL-6 and IgE in serum samples of the three groups were compared, and the differences were statistically significant (P < 0.05). Specifically, the levels of CysLTs, IL-13 and IgE in the infection group were higher than those in the non-infection group and the control group (P < 0.05), while the levels of CysLTs, IL-13 and IgE in the non-infection group were even higher than those in the control group (P < 0.05). Logistic regression analysis showed that CysLTs [ O^R=1.012 (95% CI: 1.008, 1.025) ], IL-13 [ O^R = 2.301 (95% CI: 1. 563, 3.405) ], and IgE [ O^R = 3.921 (95% CI: 1.873, 8.614) ] levels were risk factors for Mycoplasma pneumoniae infection in children with bronchial asthma (P < 0.05). In the infection group, the level of CysLTs was positively correlated with the levels of IL-13 (r = 0.979, P = 0.000) and IgE (r = 0.992, P = 0.012), and the level of IL-13 was positively correlated with that of IgE (r = 0.931, P = 0.000).Conclusions The expression levels of CysLTs, IL-13 and IgE are significantly elevated in children with bronchial asthma complicated with Mycoplasma pneumoniae infection.