ALDH2基因G487A位点多态性与2型糖尿病患者脑梗死风险的关系

The relationship between gene polymorphism of acetaldehyde dehydrogenase 2 and risk of cerebral infarction in patients with type 2 diabetes mellitus

摘要:

目的 探究2型糖尿病患者脑梗死风险与ALDH2基因G487A位点多态性的关系。方法 选取2019年1月—2020年1月海口市人民医院收治的116例2型糖尿病患者为研究对象，根据是否发生脑梗死分为脑梗死组71例和非脑梗死组45例。采用限制性片段长度多态性聚合酶链反应（PCR-RELP）检测受试者血清ALDH2基因G487A位点多态性分布频率，并分析ALDH2基因单核苷酸各基因型与脑梗死风险的关系。结果 ALDH2基因G487A位点基因型有野生GG型、突变杂合子GA型、突变纯合子AA型。与非脑梗死组患者比较，脑梗死组患者总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）水平较高（P <0.05），脑梗死组G487A位点AG型、AA型及A等位基因频率较高（P <0.05）。多因素Logistic回归分析显示，TG［R=2.396（95% CI：1.029，5.531）］、LDL-C［R=2.512（95% CI：1.012，3.238）］、ALDH2基因G487A位点（以野生GG型为参照）杂合子AG型［R=3.107（95% CI：1.456，6.634）］、纯合子突变AA型［R=3.464（95% CI：1.594，7.528）］是2型糖尿病患者发生脑梗死的独立危险因素（P <0.05）。结论 ALDH2基因G487A位点多态性与2型糖尿病患者发生脑梗死有关。

Abstract:

Objective To investigate the relationship between G487A polymorphism in acetaldehyde dehydrogenase 2 (ALDH2) gene and risk of cerebral infarction in patients with type 2 diabetes mellitus.Methods A total of 116 patients with type 2 diabetes mellitus admitted to our hospital from January 2019 to January 2020 were selected and divided into cerebral infarction group (71 cases) and non-cerebral infarction group (45 cases) according to the occurrence of cerebral infarction. The distribution frequency of G487A polymorphism in ALDH2 gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), and the relationship between the single nucleotide polymorphism genotypes of ALDH2 gene and the risk of cerebral infarction was analyzed.Results The genotypes of G487A locus in ALDH2 gene include wild type (GG), heterozygous mutant (GA) and homozygous mutant (AA). The levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein-cholesterol (LDL-C) of patients in cerebral infarction group were higher than those in non-cerebral infarction group (P < 0.05), and the frequency of AG genotype, AA genotype and A allele at G487A locus was significantly higher in the cerebral infarction group (P < 0.05). Multivariate Logistic regression analysis showed that TG [R = 2.396 (95% CI: 1.029, 5.531)], LDL-C [R = 2.512 (95% CI: 1.012, 3.238)], AG genotype (versus GG genotype) [R = 3.107 (95% CI: 1.456, 6.634)], and AA genotype [R = 3.464 (95% CI: 1.594, 7.528)] were independent risk factors for cerebral infarction in type 2 diabetic patients (P < 0.05).Conclusions The G487A polymorphism in ALDH2 gene is related to the occurrence of cerebral infarction in type 2 diabetic patients.