MDR1、PEDF、LRP在宫颈癌中的表达及意义

Expression of MDR1, PEDF, and LRP in cervical cancer and its clinical significance

摘要:

目的 探讨宫颈癌组织中多药耐药基因（MDR1）、色素上皮源性因子（PEDF）和肺耐药蛋白（LRP）阳性率和mRNA的表达及临床意义。方法 选取2014年1月—2015年12月在安徽医科大学附属安庆医院就诊的宫颈癌患者58例（宫颈癌组）和子宫良性病变行子宫切除术患者9例（对照组）。采用RT-PCR检测宫颈癌组织和正常宫颈组织中MDR1、PEDF和LRP mRNA相对表达量；采用免疫组织化学法检测宫颈癌组织和正常宫颈组织中MDR1、PEDF和LRP的蛋白阳性表达率。结果 宫颈癌组织中MDR1和LRP mRNA相对表达量高于正常宫颈组织（P <0.05），PEDF mRNA相对表达量低于正常宫颈组织（P <0.05）；宫颈癌组织中MDR1和LRP蛋白阳性表达率高于正常宫颈组织（P <0.05），PEDF蛋白阳性表达率低于正常宫颈组织。MDR1和LRP高表达的宫颈癌患者5年总生存率低于MDR1和LRP低表达的宫颈癌患者（P <0.05）；PEDF高表达的宫颈癌患者5年生存率高于PEDF低表达的宫颈癌患者（P <0.05）。结论 MDR1和LRP在宫颈癌中高表达，PEDF在宫颈癌中低表达，且与临床病理特征及预后有关。

Abstract:

Objectives To investigate the mRNA and protein expression of MDR1, PEDF, and LRP in cervical cancer and its clinical significance.Methods The mRNA and protein expression of MDR1, PEDF, and LRP were determined by RT-PCR and immunohistochemistry in 9 healthy people and 58 patients of cervical cancer.Results The mRNA and proteinexpression of MDR1 and LRP were higher in cervical cancer tissues than in normal cervical tissues; the mRNA and protein expression of PEDF were lower in cervical cancer tissues than in normal cervical tissues; the 5-year overall survival rate of cervical cancer patients with high expression of MDR1 and LRP was lower than that of cervical cancer patients with low expression of MDR1 and LRP, and the 5-year overall survival rate of cervical cancer patients with high expression of PEDF was higher than that of cervical cancer patients with low expression of PEDF.Conclusion MDR1 and LRP were highly expressed in cervical cancer, while PEDF was poorly expressed in cervical cancer, which was related to clinicopathological features and prognosis.