上皮性卵巢癌组织中microRNA-375、ERBB2的表达及与临床病理特征和预后的关系

Expression of miR-375 and ERBB2 in epithelial ovarian cancer and their relationship with clinicopathological parameters and prognosis

摘要:

目的 探究上皮性卵巢癌（EOC）组织中microRNA-375（miR-375）、erb-b2受体酪氨酸激酶2（ERBB2）的表达及与临床病理特征和预后的关系。方法 选取2015年10月—2017年10月上海交通大学附属第六人民医院收治的134例EOC患者的基本资料。采用实时荧光定量PCR（qRT-PCR）检测miR-375和ERBB2在EOC组织及其癌旁正常组织中的表达，并分析两者的相关性及其与临床病理特征和预后的关系。结果 EOC组织中miR-375 mRNA相对表达量较癌旁组织低（P <0.05），而ERBB2 mRNA相对表达量较癌旁组织高（P <0.05）。EOC组织中miR-375 mRNA和ERBB2 mRNA相对表达量在淋巴结转移和FIGO分期方面差异有统计学意义（P <0.05）。EOC组织中miR-375的表达与ERBB2的表达呈负相关（P <0.05）。miR-375低表达组的3年总生存率较高表达组低（P <0.05）。ERBB2高表达组的3年总生存率较低表达组低（P <0.05）。Cox回归分析显示，高级别的FIGO分期、有淋巴结转移、miR-375低表达以及ERBB2高表达是影响EOC患者预后的独立危险因素（P <0.05）。结论 EOC组织中miR-375呈低表达而ERBB2呈高表达，两者呈负相关，且均与FIGO分期及淋巴结转移有关，对评估病情进展及其预后生存率具有重要的临床价值。

Abstract:

Objective To investigate the expression of miR-375 and erb-b2 receptor tyrosine kinase 2 (ERBB2) in epithelial ovarian cancer (EOC) and their relationship with clinicopathological parameters and prognosis.Methods The basic data of 134 EOC patients admitted to The Sixth People's Hospital Affiliated to Shanghai Jiaotong from October 2015 to October 2017 were selected. The expression of miR-375 and ERBB2 in EOC tissues and adjacent normal tissues was determined by real-time quantitative PCR (qRT-PCR), and the relationship between the two index and their clinicopathological features and prognosis was analyzed.Results The expression of miR-375 in EOC tissues was lower than that in adjacent tissues, while the expression of ERBB2 was higher than that in adjacent tissues (all P < 0.05). Both miR-375 and ERBB2 expression were related with FIGO stage and lymph node metastasis (all P <0.05). The expression level of miR-375 in EOC tissues was negatively correlated with the expression level of ERBB2 (P < 0.05). The 3-year overall survival rate of low miR-375 expression group were lower than those of high expression group (P < 0.05). The 3-year overall survival rate of high ERBB2 expression group were lower than those of low expression group (P < 0.05). Cox regression analysis showed that high-level FIGO stage, lymph node metastasis, low miR-375 expression, and high ERBB2 expression were independent risk factors for the prognosis of EOC patients (all P < 0.05).Conclusions The expression of miR-375 is low and ERBB2 is high in EOC. There is a negative correlation between them, and both of them are related to FIGO stage and lymph node metastasis, which have important clinical value in evaluating disease progression and survival rate.