甘草酸对大鼠急性肺水肿的影响

Effect of glycyrrhizin on acute pulmonary edema in rat models

摘要:

目的 探讨甘草酸对大鼠急性肺水肿呼吸指数的调控。方法 将18只大鼠随机分为正常组、模型组、甘草酸组。大鼠腹腔注射6%氯化铵复制急性肺水肿模型，通过肺功能仪测定大鼠的肺功能指标，湿重/干重衡量水肿程度，HE染色观察肺组织病理损伤情况。结果 模型组肺功能残气量、最大变化压力、最大变化体积、低于正常组和甘草酸组（P <0.05）。各组大鼠阻塞时间比较，差异无统计学意义（P >0.05）。模型组弦性顺应性、深吸气量、肺活量低于正常组、甘草酸组（P <0.05）。模型组最大呼气流速、用力呼吸残气量、100毫秒用力呼气容积高于正常组（P <0.05），但低于甘草酸组（P <0.05），模型组用力肺活量低于正常组、甘草酸组（P <0.05）。各组大鼠20毫秒用力呼气容积比较，差异无统计学意义（P >0.05）。模型组肺组织含水率高于正常组、甘草酸组（P <0.05）。HE染色结果显示，肺组织水肿程度明显降低，肺泡间隔缩小。结论 甘草酸可通过改善肺通气量，减弱肺表面的水肿程度来缓解大鼠急性肺水肿，减少炎症细胞浸润。

Abstract:

Objective To explore the role of glycyrrhizin in regulating the respiratory index of rats with acute pulmonary edema.Methods Eighteen rats were randomly divided into normal group, model group and glycyrrhizin group. Rats were intraperitoneally injected with 6% ammonium chloride to establish the acute pulmonary edema models. The pulmonary function parameters of the rats were measured. The degree of edema was measured by wet/dry weight ratio. The pathological damage of lung tissues was observed by H&E staining.Results The FRC, DP_max, and DV_max were lower in the model group than those in the normal group and glycyrrhizin group (P < 0.05). There was no difference in the Tocclude among the groups (P > 0.05). The Cchord, IC, and VC were lower in the model group than those in the normal group and glycyrrhizin group (P < 0.05). The PEF, FERV, and FEV_0.1 were higher in the model group than those in the normal group, but were lower than those in the glycyrrhizin group (P < 0.05). The FVC in the model group was lower than that in the normal group and glycyrrhizin group (P < 0.05). There was no difference in the FEV_0.02 among the groups (P > 0.05). The wet/dry weight ratio of the lung tissues was higher in the model group than that in the normal group and glycyrrhizin group (P < 0.05). After H&E staining, glycyrrhizin altered the edema fluid and cellular components in the lung tissues, reduced the number of red blood cells, and narrowed the alveolar septa.Conclusions The glycyrrhizin ameliorates the acute pulmonary edema in rats by enhancing the vital capacity and reducing the degree of edema on the lung surface as evaluated via the spirometry. Besides, the infiltration of inflammatory cells can also be effectively mitigated.