乳腺癌组织miR-92a、KLF4 mRNA的表达及与临床病理参数、预后的关系

Expressions of miR-92a and KLF4 mRNA in breast cancer tissues and their relationship with pathological parameters and prognosis

摘要:

目的 探讨乳腺癌组织中miRNA-92a、KLF4 mRNA的表达及与临床病理参数、预后的关系。方法 选取2014年1月—2016年9月长沙市第一医院乳甲外科收治的124例乳腺癌患者。qRT-PCR检测癌组织和距离> 5 cm的癌旁组织中miR-92a、KLF4 mRNA表达；Pearson相关性分析乳腺癌组织中miR-92amRNA表达与KLF4 mRNA表达的相关性；绘制不同miR-92a、KLF4 mRNA表达乳腺癌患者的生存曲线；多因素Cox回归分析乳腺癌患者预后不良影响因素。结果 癌组织miR-92a、KLF4 mRNA相对表达量高于癌旁组织（P <0.05）。Pearson相关性分析显示，乳腺癌组织中miR-92a与KLF4 mRNA表达呈正相关（r =0.612，P <0.05）。临床分期Ⅲ、Ⅳ期和有淋巴结转移患者的miR-92a、KLF4 mRNA表达水平高于临床分期Ⅰ、Ⅱ期和无淋巴结转移患者（P <0.05）。不同年龄、肿瘤直径、分化程度、雌激素受体、孕激素受体患者miR-92a、KLF4 mRNA相对表达量比较，差异无统计学意义（P >0.05）。miR-92a、KLF4 mRNA相对表达量高表达患者累积生存率低于低表达患者（P <0.05）。多因素Cox回归分析显示，临床分期Ⅲ、Ⅳ期［H^R=3.716（95% CI：1.765，7.826）］、淋巴结转移［H^R=3.021（95% CI：1.341，6.803）］、miR-92a ≥ 1.627［H^R=3.401（95% CI：1.358，8.515）］、KLF4 mRNA≥ 2.270［H^R=2.059（95% CI：1.026，4.133）］是乳腺癌患者预后不良的危险因素（P <0.05）。结论 乳腺癌组织中miR-92a、KLF4 mRNA高表达与临床分期、淋巴结转移有关，可能成为乳腺癌预后的标志物。

Abstract:

Objective To investigate the expressions of microRNA (miRNA)-92a and Krüppel-like factor 4 (KLF4) mRNA in breast cancer tissues and their relationship with pathological parameters and prognosis.Methods A total of 124 breast cancer patients admitted to our hospital from January 2014 to September 2016 were selected. The expressions of miR-92a and KLF4 mRNA in cancer tissues and adjacent tissues more than 5 cm away from the tumor edge were detected by qRT-PCR. Pearson correlation analysis was used to investigate the correlation between the expression of miR-92a and that of KLF4 mRNA in breast cancer tissues. Survival curves of breast cancer patients with different expressions of miR-92a and KLF4 mRNA were plotted, and multivariable Cox regression analysis was performed to determine the factors affecting the prognosis in breast cancer patients.Results The relative expressions of miR-92a and KLF4 mRNA in breast cancer tissues were higher than those in adjacent tissues (P < 0.05). Pearson correlation analysis showed that there was a positive correlation between miR-92a and KLF4 mRNA expression in breast cancer tissues (r = 0.612, P < 0.05). The expression levels of miR-92a and KLF4 mRNA in patients with stage Ⅲ and Ⅳ tumors as well as lymph node metastasis were higher than those in patients with stage Ⅰ and Ⅱ and without lymph node metastasis (P < 0.05). There was no significant difference in the expression levels of miR-92a and KLF4 mRNA in patients with different age, tumor diameter, degree and of differentiation, and the in those with breast cancer positive or negative for estrogen receptor and progesterone receptor (P > 0.05). The accumulative survival rates of patients with high expressions of miR-92a and KLF4 mRNA were lower than those of patients with low expressions of miR-92a and KLF4 mRNA. Multivariable Cox regression analysis showed that stage IV and III tumors [H^R = 3.716, (95%CI: 1.765 ,7.826) ], lymph node metastasis [H^R = 3.021, (95% CI: 1.341, 6.803) ], miR-92a ≥ 1.627 [H^R = 3.401, (95% CI: 1.358, 8.515) ], and KLF4 mRNA≥ 2.270 [H^R = 2.059, (95% CI: 1.026, 4.133) ] were risk factors for poor prognosis in breast cancer patients (P < 0.05).Conclusions High expressions of miR-92a and KLF4 mRNA in breast cancer tissues are associated with TNM stage and lymph node metastasis, and may be potential biomarkers for assessing breast cancer prognosis.