金芪灌洗液对慢性鼻-鼻窦炎模型白兔鼻黏膜组织miRNA-155-3p表达的影响

Effects of Jinqi for nasal lavage on the expression of miRNA-155-3p in nasal mucosa of rabbit models with chronic rhinosinusitis

摘要:

目的 探讨中药金芪灌洗液对慢性鼻-鼻窦炎（CRS）模型白兔鼻黏膜组织miRNA-155-3p表达的影响。方法 从慢性鼻-鼻窦炎患者的鼻腔分泌物中单独分离出金黄色葡萄球菌菌株，培养后收集菌落并稀释至1.0×10¹¹ cfu/L混悬液，通过手术方式移植到白兔的鼻腔内，复制慢性鼻-鼻窦炎动物模型。将实验白兔随机分为空白组、模型组、庆大霉素组、金芪灌洗液组，每组6只。各组白兔给予相应干预，观察2周后处死取材。采用实时荧光定量聚合酶链反应检测miRNA-155-3p的表达，HE染色观察各组鼻黏膜炎症及结构的变化。结果 模型组、庆大霉素组miRNA-155-3p相对表达量较空白组升高（P <0.05），金芪灌洗液组较空白组降低（P <0.05），金芪灌洗液组miRNA-155-3p相对表达量较模型组降低（P <0.05），庆大霉素组较模型组升高（P <0.05），金芪灌洗液组miRNA-155-3p相对表达量较庆大霉素组降低（P <0.05）。白兔鼻窦黏膜形态学改变显示金芪灌洗液组黏膜形态趋于正常。结论 金芪灌洗液可抑制miRNA-155-3p的表达，起到抗炎作用并修复白兔损伤的鼻黏膜。

Abstract:

Objective To investigate the effects of traditional Chinese medicine Jinqi for nasal lavage on expressions of inflammatory genes in nasal mucosa in chronic rhinosinusitis.Methods Staphylococcus aureus strains were isolated and screened from the nasal secretions of patients with chronic rhinosinusitis. After culture, the colonies were collected and serially diluted to 1.0×10¹¹ cfu/L suspension, which was surgically implanted into the nasal cavity of rabbits to establish an animal model of chronic rhinosinusitis. The rabbits were then randomly divided into a blank group, a model group, a gentamicin group, and a Jinqi lavage group, with 6 rabbits in each group. The rabbits of each group were treated respectively and were sacrificed after 2 weeks of observation. The quantitative real-time polymerase chain reaction was used to detect the expression of miRNA-155-3p, and H&E staining was used to observe the histopathological changes of the nasal mucosa in each group.Results Compared with the blank group, the expression of miRNA-155-3p was higher in the model group and gentamicin group (P < 0.05), while that in the Jinqi lavage group was lower (P < 0.05). The expression of miRNA-155-3p in the Jinqi lavage group was lower and that in the gentamicin group was higher compared with the model group (P < 0.05). Besides, the expression of miRNA-155-3p in the Jinqi lavage group was even lower than that in the gentamicin group (P < 0.05). The morphology of the nasal mucosa of rabbits in the Jinqi lavage group was nearly normal.Conclusions Jinqi for nasal lavage shows an inhibitory effect on the expression of miRNA-155-3p, thereby playing an anti-inflammatory role and repairing the damaged nasal mucosa.