妊娠期糖尿病患者血清microRNA-122、血管内皮生长因子的表达及与围生儿结局的关系

Serum levels of miR-122 and VEGF in patients with gestational diabetes mellitus and their relationship with perinatal outcome

摘要:

目的 分析妊娠期糖尿病（GDM）患者血清microRNA-122（miR-122）、血管内皮生长因子（VEGF）表达及与围生儿结局的关系。方法 选取2018年5月—2020年1月川北医学院附属医院妇产科收治的GDM患者80例作为研究组，另选取同期在该院产检的健康妊娠女性70例作为对照组。比较两组血糖、miR-122、VEGF，分析血糖水平与miR-122、VEGF的相关性；对比不同围生儿结局患者的miR-122、VEGF，绘制ROC曲线分析miR-122、VEGF预测围生儿结局的价值。结果 研究组血糖、miR-122、VEGF高于对照组（P <0.05）。不良组miR-122、VEGF高于良好组（P <0.05）。Pearson相关分析结果显示，GDM患者miR-122与FGB、2 hPG和HbA1c呈正相关（r =0.605、0.752和0.542，均P <0.05），VEGF与FGB、2 hPG和HbA1c呈正相关（r =0.532、0.623和0.596，均P <0.05）。ROC曲线结果显示，miR-122、VEGF及两者联合检测GDM患者不良围产儿结局的AUC分别为0.787（95% CI：0.687，0.887）、0.755（95% CI：0.658，0.851）、0.842（95% CI：0.760，0.923）。结论 GDM患者血清miR-122、VEGF均存在表达失调的情况，两者与血糖呈正相关，且联合检测可有效预测不良围生儿结局。

Abstract:

Objective To analyze the expressions of miR-122 and vascular endothelial growth factor (VEGF) in serum of patients with gestational diabetes mellitus (GDM) and their relationship with perinatal outcome.Methods A total of 80 patients with GDM admitted from May 2018 to January 2020 in the Department of Obstetrics and Gynecology of the Affiliated Hospital of North Sichuan Medical College were selected as the study group. In addition, 70 healthy pregnant women receiving the antenatal care in the hospital during the same period were included as the control group. The levels of blood glucose, miR-122 and VEGF were compared between the two groups, and the correlation between the levels of blood glucose and those of miR-122 and VEGF was analyzed. The levels of miR-122 and VEGF were also compared between patients with different perinatal outcomes, and the value of miR-122 and VEGF in predicting perinatal outcomes was determined by receiver operating characteristic (ROC) curve analysis.Results The levels of blood glucose, miR-122 and VEGF were higher in the study group than those in the control group (P < 0.05), and the levels of miR-122 and VEGF in patients with poor perinatal outcomes were higher compared with those with favorable perinatal outcomes (P < 0.05). Pearson correlation analysis showed that the levels of miR-122 (r = 0.605, 0.752 and 0.542, all P <0.05) and VEGF (r =0.532, 0.623 and 0.596, all P <0.05) were positively correlated with FGB, 2 hPG and HbA1c. As demonstrated in the ROC curve analysis, the area under the ROC curve (AUC) of miR-122, VEGF and the combination of the two indicators for predicting adverse perinatal outcomes in GDM patients were 0.787 (95% CI: 0.687, 0.887), 0.755 (95% CI: 0.658, 0.851), and 0.842 (95% CI: 0.760, 0.923), respectively.Conclusions Serum miR-122 and VEGF are dysregulated in GDM patients and they are positively correlated with the level of blood glucose. The combined detection of serum miR-122 and VEGF can effectively predict the occurrence of adverse perinatal outcomes.