干扰素诱导蛋白16在胎膜早破孕妇血清、胎盘组织中的表达及其意义

Expression and clinical significance of interferon-inducible protein 16 in the serum and placental tissues of pregnant women with premature rupture of membranes

摘要:

目的 研究孕妇血清、胎盘组织中γ干扰素诱导蛋白16（IFI16）表达水平与胎膜早破（PROM）的关系，为感染性PROM的早期诊断提供参考。方法 选取2019年9月—2020年9月徐州医科大学附属徐州妇幼保健院收治的PROM手术终止妊娠孕妇60例作为研究组。依据孕周将研究组分为足月PROM组［（38.16±1.47）周］和未足月PROM组［（34.87±2.05）周］，各30例。选取同期该院足月正常剖宫产的产妇30例作为对照组。对比3组胎盘组织IFI16表达情况，IFI16 mRNA、Caspase-1 mRNA表达水平及孕妇血清IFI16、炎症因子［白细胞介素-6（IL-6）、IL-1β］水平。结果 足月PROM组、未足月PROM组IFI16蛋白阳性率高于对照组（P <0.05），足月PROM组与未足月PROM组IFI16蛋白阳性率比较，差异无统计学意义（P >0.05）。未足月PROM组、足月PROM组胎盘组织IFI16 mRNA、Caspase-1 mRNA相对表达量较对照组高（P <0.05）。未足月PROM组、足月PROM组血清IFI16、IL-6、IL-1β水平较对照组高（P <0.05）。结论 IFI16在孕妇血清及胎盘组织中升高可能与PROM孕妇的炎症反应有关，IFI16水平升高对PROM炎症反应可能有预测意义。

Abstract:

Objective To investigate the relationship between the expression levels of interferon-inducible protein 16 (IFI16) in the placental tissues and maternal blood and the onset of premature rupture of membranes (PROM), so as to provide references for the early diagnosis of infection-associated PROM.Methods We included 60 patients with PROM undergoing the operation to terminate the pregnancy who were admitted to Xuzhou Maternal and Child Health Hospital from September 2019 to September 2020. According to the gestational week when PROM occurred, the patients were divided into full-term PROM group (37 weeks to 42 weeks of pregnancy, 30 cases) and preterm PROM group (28 weeks to 36 weeks and 6 days of pregnancy, 30 cases). Thirty women with full-term pregnancy receiving cesarean sections during the same period were enrolled as the control group. The mRNA and protein expressions of IFI16, and mRNA expression of Caspase-1 in placental tissues, as well as the expression levels of IFI16 and inflammatory factors including interleukin (IL)-6 and IL-1β in the serum of pregnant women were compared among the three groups.Results The positive rate of IFI16 expression in the placental tissues of pregnant women in the full-term PROM group and the preterm PROM group was higher than that in the control group (P < 0.05), while there was no significant difference in that between the full-term PROM group and the preterm PROM group (P > 0.05). The mRNA expressions of IFI16 and Caspase-1 in placental tissues of pregnant women in the full-term PROM group and the preterm PROM group were higher than those in the control group (P < 0.05). In addition, the maternal serum levels of IFI16, IL-6 and IL-1β in the full-term PROM group and the preterm PROM group were higher than those in the control group (P < 0.05).Conclusions The elevated level of IFI16 in maternal blood and placental tissues may be related to inflammatory responses in PROM. The detection of IFI16 may be of predictive significance for the inflammatory responses in PROM.