心肌缺血再灌注损伤模型复制方法的研究进展

Research progress in establishing myocardial ischemia-reperfusion injury model

摘要:

复制心肌缺血再灌注损伤模型是进行心肌缺血再灌注损伤研究的基础，复制稳定且高效的动物和细胞模型尤为重要。目前，心肌缺血再灌注损伤模型分为结扎动物冠状动脉左前降支的在体模型、动物心脏灌流装置及细胞缺氧再灌注的离体模型，但在模型复制过程中仍存在成功率低、重复性差等问题。因此，该文结合近年研究对该模型的复制和改良方法进行归纳和综述，为复制更为稳定的模型提供新的思路和方向。

Abstract:

The establishment of myocardial ischemia-reperfusion injury model is the basis of myocardial ischemia-reperfusion injury research. It is particularly important to establish stable and efficient animal and cell models. At present, myocardial ischemia-reperfusion injury model can be divided into in vivo model of ligation of animal anterior descending coronary artery, animal heart perfusion device and in vitro model of cell hypoxia reperfusion. However, there are still some problems in model construction, such as low success rate and poor repeatability. Consequently, this paper summarizes and reviews the construction and improvement methods of this model based on recent studies, providing new ideas and directions for the construction of a more stable model.