MicroRNA-183-5p在动脉粥样硬化患者中的表达及其临床意义

Expression and clinical significance of miR-183-5p in patients with atherosclerosis

摘要:

目的 分析microRNA-183-5p（miR-183-5p）在动脉粥样硬化患者中的表达及其临床意义。方法 选取2021年1月—2022年8月四川省人民医院收治的经血管造影确诊的103例动脉粥样硬化患者为研究组，选取同期该院年龄相当的90例健康体检者为健康组。采用实时荧光定量聚合酶链反应（qRT-PCR）检测两组患者血清miR-183-5p的表达，绘制受试者工作特征（ROC）曲线，分析miR-183-5p在动脉粥样硬化中的诊断价值。结果 研究组血清miR-183-5p mRNA相对表达量高于健康组（P <0.05）；研究组中高Gensini积分组患者血清miR-183-5p mRNA相对表达量高于低Gensini积分组（P <0.05）；Pearson相关性分析结果显示，miR-183-5p与C反应蛋白、颈动脉内中膜厚度及Gensini积分呈正相关（r =0.664、0.571和0.712，均P <0.05）；ROC曲线分析结果显示，miR-183-5p诊断动脉粥样硬化的截断值为2.08，敏感性和特异性分别为87.8%（95% CI：0.832，0.924）和91.6%（95% CI：0.877，0.955）。结论 动脉粥样硬化患者血清miR-183-5p高表达，能够辅助区分冠状动脉狭窄程度，可作为动脉粥样硬化临床诊断的重要生物标志物。

Abstract:

Objective To analyze the expression and clinical significance of miR-183-5p in patients with atherosclerosis.Methods A total of 103 atherosclerotic patients diagnosed by angiography admitted to Sichuan Provincial People's Hospital from January 2021 to August 2022 were selected as the study group, and 90 age-matched healthy people undergoing health checkup in the hospital during the same period were selected as the healthy group. The expression level of miR-183-5p in the serum of the two groups was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the diagnostic value of serum miR-183-5p for atherosclerosis was determined with the receiver operating characteristic curve (ROC curve).Results The relative expression of serum miR-183-5p in the study group was higher than that in the healthy group (P < 0.05). Within the study group, the relative expression of serum miR-183-5p in those with high Gensini score was higher than that in those with low Gensini score (P < 0.05). Pearson correlation analysis showed that the relative expression of miR-183-5p was positively correlated with the level of C-reactive protein (CRP), carotid intima-media thickness (CIMT) and Gensini scores (r = 0.664, 0.571 and 0.712, all P < 0.05). The ROC curve analysis revealed that the optimal cut-off value of miR-183-5p was 2.08 for diagnosing atherosclerosis, with the sensitivity and specificity being 87.8% (95% CI: 0.832, 0.924) and 91.6% (95% CI: 0.877, 0.955), respectively.Conclusions Serum miR-183-5p is highly expressed in patients with atherosclerosis, and it can help distinguish the degree of coronary artery stenosis and can be established as an important biomarker for clinical diagnosis and prognosis evaluation of atherosclerosis.