幽门螺杆菌对慢性阻塞性肺疾病大鼠气道黏膜炎症的影响

Effects of Helicobacter pylori on airway mucosal inflammation in COPD rats

摘要:

目的 探讨幽门螺杆菌（Hp）感染对慢性阻塞性肺疾病（COPD）大鼠气道炎症因子的影响。方法 选取25只8周Wistar大鼠，随机分为COPD+Hp组11只，COPD组11只，空白对照组3只。采用NaHCO₃联合Hp复制Hp感染大鼠模型。香烟烟熏联合气管内滴注脂多糖方法复制COPD大鼠模型。模型复制后4周，收集COPD+Hp组、COPD组大鼠的支气管肺泡灌洗液（BALF），采用酶联免疫吸附试验测定大鼠BALF的炎症因子白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）水平。结果 COPD组大鼠BALF的IL-6、IL-8、TNF-α水平分别为（51.20±14.13）、（103.33±15.36）和（150.40±19.82） pg/mL，COPD+Hp组大鼠BALF的IL-6、IL-8、TNF-α水平分别为（87.51±7.66）、（188.73±6.83）和（227.78±33.16） pg/mL，COPD+Hp组大鼠BALF的IL-6、IL-8、TNF-α水平均高于COPD组（P <0.05）。结论 Hp可导致COPD大鼠气道炎症因子水平升高，加重气道炎症。

Abstract:

Objective To clarify the effect of Helicobacter pylori (Hp) infection on airway inflammatory factors in rats with chronic obstructive pulmonary disease (COPD).Methods Twenty-five wistar rats at 8 weeks were randomly divided into 3 groups: 1.Hp+ COPD dual model group (11 rats); 2.COPD group (11 rats); 3.blank control group (3 rats). The group of Hp infection was established by NaHCO3 combined with Hp. The group of COPD was established by cigarette smoking combined with intratracheal infusion of lipopolysaccharide. Four weeks after the modeling, the bronchoalveolar lavage fluid (BALF) of COPD + Hp group and COPD group were collected, and the levels of inflammatory factors interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) were measured with ELISA.Results The levels of IL-6, IL-8 and TNF-α in COPD + Hp group were (87.51 ± 7.66) pg/mL,（188.73 ± 6.83) pg/mL and (227.78 ± 33.16) pg/mL, respectively, and the levels were (51.20 ± 14.13) pg/mL, (103.33 ± 15.36) pg/mL and (150.40 ± 19.82) pg/mL in COPD group. The levels of IL-6, IL-8 and TNF-α in COPD + Hp group were higher than those in COPD group (P < 0.05).Conclusion Helicobacter pylori infection can increase the level of airway inflammatory factors and aggravate airway inflammation in COPD rats.