白藜芦醇对脑小血管疾病大鼠神经细胞损伤及免疫因子白细胞介素-6、白细胞介素-10水平的影响

Effects of resveratrol on neuronal cell injury and levels of immune factors IL-6 and IL-10 in rats with cerebral small vessel disease

摘要:

目的 探究白藜芦醇对脑小血管疾病（CSVD）大鼠神经细胞损伤及免疫因子白细胞介素-6（IL-6）、白细胞介素-10（IL-10）水平的影响。方法 将60只CSVD大鼠分为假手术组、模型组和治疗组，每组20只。通过水迷宫实验测试大鼠神经认知功能，HE染色观察大鼠海马组织病理学改变，TUNEL染色检测大鼠海马组织神经细胞凋亡率，Western blotting检测大鼠海马组织BAX/BCL-2、IL-6、IL-10蛋白的表达，酶联免疫吸附试验（ELISA）检测外周血IL-6、IL-10、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）和丙二醛（MDA）水平。结果 模型组大鼠神经行为评分高于治疗组（P <0.05）。模型组大鼠穿越次数和停留时间少于假手术组（P <0.05），治疗组大鼠穿越次数和停留时间较模型组增加（P <0.05）。模型组大鼠海马组织神经细胞凋亡率、BAX/BCL-2蛋白表达比值高于假手术组（P <0.05），治疗组大鼠海马组织神经细胞凋亡凋亡率、AX/BCL-2蛋白表达比值低于模型组（P <0.05）。与假手术组比较，模型组大鼠外周血SOD、GSH、CAT降低（P <0.05），MDA升高（P <0.05）；与模型组比较，治疗组大鼠外周血SOD、GSH、CAT升高（P <0.05），MDA降低（P <0.05）。与假手术组比较，模型组大鼠外周血和海马组织免疫因子IL-6升高（P <0.05），IL-10降低（P <0.05）。与模型组比较，治疗组大鼠外周血和海马组织免疫因子IL-6降低（P <0.05），IL-10升高（P <0.05）。结论 白藜芦醇能够提高CSVD大鼠神经认知功能，改善海马组织损伤，抑制神经细胞凋亡，其作用可能与调节免疫因子IL-6、IL-10水平有关。

Abstract:

Objective To investigate the effects of resveratrol on neural cell injury and the levels of immune factors interleukin (IL)-6 and IL-10 in rats with cerebral small vessel disease (CSVD).Methods Sixty CSVD rat models were divided into sham-operation group, model group and treatment group, with 20 rats in each group. The neurocognitive functions of rats were determined by water maze tests. The histopathological changes of rat hippocampus were analyzed by hematoxylin and eosin staining, and the apoptosis rate of neuronal cells within rat hippocampal tissues was measured by TUNEL staining. The protein levels of BAX/BCL-2, IL-6 and IL-10 were detected by Western blotting, while the levels of IL-6, IL-10, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and malonaldehyde (MDA) in the peripheral blood were detected via enzyme-linked immunosorbent assay (ELISA).Results The neurobehavioral scores of rats in the model group were higher than those in the treatment group (P < 0.05). The number of platform crossings was lower and time spent in the target quadrant was shorter in the model group than in the sham-operation group (P < 0.05), while the number of platform crossings was higher and time spent in the target quadrant was longer in the treatment group than in the model group (P < 0.05). The apoptosis rates of neuronal cells within rat hippocampal tissues and the ratio of the protein level of BAX to that of BCL-2 were higher in the model group compared with the sham-operation group (P < 0.05), while those in the treatment group were lower compared with the model group (P < 0.05). In comparison to those in the sham-operation group, the levels of SOD, GSH, and CAT were lower, and the level of MDA was higher in the peripheral blood of rats in the model group (P < 0.05). The levels of SOD, GSH, and CAT were higher, and the level of MDA was lower in the peripheral blood of rats in the treatment group relative to those in the model group (P < 0.05). Compared with the sham-operation group, the level of IL-6 was higher while that of IL-10 was lower in the peripheral blood and hippocampal tissues of rats in the model group compared with the sham-operation group (P < 0.05). Besides, the level of IL-6 was lower while that of IL-10 was higher in the peripheral blood and hippocampal tissues of rats in the treatment group compared with the model group (P < 0.05).Conclusions Resveratrol improves the neurocognitive function, ameliorates the injury of hippocampal tissues and inhibits neuronal apoptosis in CSVD rats, and these effects may be mediated via the regulation of the levels of immune factors IL-6 and IL-10.