经皮神经电刺激对睡眠剥夺小鼠疲劳状态的影响

Effects of transcutaneous electrical nerve stimulation on fatigue status in sleep deprived mice

摘要:

目的 分析经皮神经电刺激（TENS）治疗对睡眠剥夺小鼠疲劳状态的影响及可能的神经体液机制。方法 SPF级昆明小鼠共18只，按照随机数字表法分为电刺激+睡眠剥夺组、睡眠剥夺组及正常组，每组6只。电刺激+睡眠剥夺组行每日睡眠剥夺20 h联合TENS干预；睡眠剥夺组行每日睡眠剥夺20 h；正常组不行任何干预。干预7 d后进行悬尾实验和旷场实验以采集相关行为学数据，并采用竞争性抑制酶免疫分析技术检测各组小鼠的血清促肾上腺皮质激素释放因子和皮质酮（CORT）水平。结果 悬尾实验结果表明，电刺激＋睡眠剥夺组的不动时间占比低于睡眠剥夺组（P <0.05），电刺激＋睡眠剥夺组的挣扎时间占比高于睡眠剥夺组（P <0.05）。旷场实验结果表明，电刺激+睡眠剥夺组的中央区域时间长于睡眠剥夺组（P <0.05）。血清激素水平检测结果显示，睡眠剥夺组的血清CORT水平高于正常组（P <0.05）。结论 TENS可改善小鼠因睡眠不足导致的焦虑、抑郁等精神行为改变，这可能与其对体内下丘脑-垂体-肾上腺轴激素水平的调节有关，TENS在改善睡眠不足导致的精神疲劳方面有潜在价值。

Abstract:

Objective To analyze the role of transcutaneous electrical nerve stimulation (TENS) in alleviating fatigue in sleep-deprived mice and to explore the potential neurohumoral mechanisms involved.Methods A total of 18 SPF-grade Kunming mice were selected and numbered according to their body weight. They were randomly divided into three groups: the electrical stimulation + sleep deprivation group, the sleep deprivation group, and the normal group, with six mice in each group. Mice in the electrical stimulation + sleep deprivation group underwent 20 hours of sleep deprivation daily, combined with TENS intervention. The sleep deprivation group received only the 20 hours of sleep deprivation, while no intervention was performed on the normal group. After 7 days of intervention, all three groups underwent tail suspension and open field tests to collect relevant behavioral data. Serum levels of corticotropin-releasing factor and corticosterone (CORT) in each group were measured using competitive inhibition Enzyme-linked Immunosorbent Assay.Results The results from the tail suspension test indicated that the percentage of immobility time in the electrical stimulation + sleep deprivation group was lower than that in the sleep deprivation group (P <0.05), while the percentage of struggling time in the electrical stimulation + sleep deprivation group was higher (P <0.05). In the open field test, the time spent in the central area by the electrical stimulation + sleep deprivation group was longer compared to the sleep deprivation group (P <0.05). Serum hormone level analysis showed that serum CORT levels in the sleep deprivation group were higher than those in the normal group (P <0.05).Conclusion TENS can ameliorate anxiety, depression, and other behavioral changes induced by sleep deprivation in mice, which may be related to abnormal hormone levels in the hypothalamic-pituitary-adrenal (HPA) axis. TENS holds potential value in relieving mental fatigue caused by sleep deprivation.