微粒皮移植联合医用自凝集水凝胶修复 家兔全层皮肤缺损的实验研究

Treatment effect of micro-skin transplantation combined with medical self-agglutination hydrogel for whole skin defect in rabbits

摘要:

目的 评价自凝集水凝胶（医用可注射聚羧甲基葡萄糖胺凝胶）的皮内刺激反应，以及该材料 与微粒皮移植联用对全层皮肤缺损的疗效。方法 依据国家标准GB/T 16886.10—2005，对该材料进行皮内 刺激反应安全性评价。麻醉家兔背部，切除3.5 cm×3.5 cm 全层皮肤。切下的皮肤裁剪成1 mm×1 mm 的微 粒皮，将微粒皮在创面上摆放，滴洒水凝胶原液于创面。通过凝胶自凝完成微粒皮固定，用制备好的异体兔 脱细胞真皮紧密覆盖创面、缝合。观察4 周，修复创面做病理观察。结果 自凝集水凝胶无潜在的皮内刺激。 凝胶联合微粒皮移植术4 周后伤口完全愈合且有毛发生长，病理切片可见皮肤创面修复，炎症细胞浸润较轻。 结论 自凝集水凝胶对局部皮肤组织无刺激。凝胶联合微粒皮移植可促使创面皮肤组织的修复再生。

Abstract:

Objective To evaluate the intradermal stimulation response of medical self-agglutination hydrogel (carboxymethyl glucosamine) and the effect of it combined with micro-skin transplantation on animal experiments. Methods According to the national standard GB/T 16886. 10-2005, intradermal stimulation test of this material was evaluated. The whole skin of 3.5*3.5 cm was excised from the back of anesthetized rabbits. The cut skin was cut into 1*1 mm fine micro-skin. Micro-skin was placed on the wound surface. The hydrogel stock solution was dripped onto the wound surface. Micro-skin was fixed by hydrogel self - coagulating, then the wound surface was tightly covered and sutured with the prepared allogenic rabbit acellular dermis. Rabbits were observed for 4 weeks, and the repaired wound surface was cut for pathological observation. Results No abnormality was found in intradermal stimulation test after 4 weeks of hydrogel combined with micro-skin transplantation. The wounds healed completely and had hair growth. Pathological findings showed that the skin of wound surface repaired and the infiltration of inflammatory cells was light. Conclusions The self-agglutination hydrogel has no irritation to local skin tissue. Hydrogel combined with micro-skin transplantation can promote the repairment and regeneration of wound skin tissue.