Talin1在子痫前期患者胎盘中的表达及其对滋养层细胞生物学行为的影响

The expression of Talin1 in the placenta of patients with preeclampsia and its influence on the biological behavior of trophoblast cells

摘要:

目的 探究踝蛋白-1（Talin1）在子痫前期患者的胎盘中的表达及其对滋养层细胞生物学行为的影响机制。方法 从22例健康妊娠孕产妇人群及20例子痫前期患者中收集胎盘组织，通过蛋白免疫印迹实验分析Talin1在两组人群中的表达差异。将人胎盘滋养层细胞（HTR8/Svneo细胞）分为6组：pcDNA3.1 NC组、pcDNA3.1 Talin1组、siRNA NC组、siRNA Talin1组、激动剂组、siRNA Talin1+抑制剂组。采用MTT实验检测HTR8/Svneo细胞的增殖；采用Transwell实验检测HTR8/Svneo细胞的侵袭与迁移；采用流式细胞术检测HTR8/Svneo细胞的凋亡；采用蛋白免疫印迹实验分析HTR8/Svneo细胞中Talin1及整合位点家族成员/β-连环蛋白（Wnt/β-catenin）通路相关蛋白的表达。结果 Talin1在子痫前期患者胎盘组织中表达增加（P <0.05）。与pcDNA3.1 NC组比较，pcDNA3.1 Talin1组的HTR8/Svneo细胞的Talin1表达增加，HTR8/Svneo细胞的增殖、侵袭及迁移能力减弱，凋亡率增加，Wnt 3α、β-catenin蛋白表达下降（P <0.05）。与siRNA NC组比较，siRNA Talin1组、siRNA Talin1+抑制剂组HTR8/Svneo细胞的Talin1表达均降低（P <0.05）。激动剂组HTR8/Svneo细胞的Talin1表达相较于siRNA NC组，差异无统计学意义（P >0.05）。与siRNA NC组比较，siRNA Talin1及激动剂组的HTR8/Svneo细胞的增殖活力、侵袭及迁移能力增强，凋亡率降低，整合位点家族成员3α（Wnt 3α）、β连环蛋白（β-catenin）蛋白表达均上调（P <0.05）。siRNA Talin1+抑制剂组HTR8/Svneo细胞与siRNA NC组的HTR8/Svneo细胞的增殖、侵袭、迁移能力、细胞凋亡率、Wnt 3α、β-catenin蛋白表达比较，差异均无统计学意义（P >0.05）。结论 Talin1在子痫前期患者胎盘组织中表达增加，抑制Talin1的表达能够通过激活Wnt/β-catenin信号轴促进滋养层细胞的增殖、迁移与侵袭，并抑制滋养层细胞凋亡。

Abstract:

Objective To investigate the expression of talin-1 (Talin1) in the placentas of patients with preeclampsia and its mechanism of influence on the biological behavior of trophoblast cells.Methods Placental tissues were collected from 22 healthy pregnant women and 20 patients with preeclampsia. The expression of Talin1 in the two groups was analyzed by Western blotting. Human trophoblast cells (HTR8/Svneo cells) were divided into six groups: pcDNA3.1 NC group, pcDNA3.1 Talin1 group, siRNA NC group, siRNA Talin1 group, agonist group, and siRNA Talin1 + inhibitor group. The proliferation of HTR8/Svneo cells was detected by MTT assay; the invasion and migration of HTR8/Svneo cells were detected by Transwell assay; the apoptosis of HTR8/Svneo cells was detected by flow cytometry; and the expression of Talin1 and Wnt/β-catenin pathway-related proteins in HTR8/Svneo cells was analyzed by Western blotting.Results The expression of Talin1 in the placentas of patients with preeclampsia was increased (P < 0.05). Compared with the pcDNA3.1 NC group, the expression of Talin1 in the pcDNA3.1 Talin1 group was increased, and the proliferation, invasion, and migration abilities of HTR8/Svneo cells were weakened, the apoptosis rate was increased, and the expression of Wnt 3α and β-catenin proteins was decreased (P < 0.05). Compared with the siRNA NC group, the expression of Talin1 in the siRNA Talin1 group and the siRNA Talin1 + inhibitor group was decreased (P < 0.05). There was no significant difference in the expression of Talin1 between the agonist group and the siRNA NC group (P > 0.05). Compared with the siRNA NC group, the proliferation, invasion, and migration abilities of HTR8/Svneo cells in the siRNA Talin1 and agonist groups were enhanced, the apoptosis rate was decreased, and the expression of wingless-type MMTV integration site family 3α (Wnt 3α) and β-catenin proteins was upregulated (P < 0.05). There was no significant difference in the proliferation, invasion, migration ability, apoptosis rate, and expression of Wnt 3α and β-catenin proteins between the siRNA Talin1 + inhibitor group and the siRNA NC group (P > 0.05).Conclusion The expression of Talin1 is increased in the placentas of preeclampsia patients. Inhibition of Talin1 expression can promote the proliferation, migration and invasion of trophoblast cells and inhibit the apoptosis of trophoblast cells by activating Wnt/β-catenin signaling axis.